Mouse anti‑Xenopus ACTB / Beta Actin IgG1 Monoclonal RW-C20276-100
Antibody: ACTB / Beta Actin Mouse anti-Xenopus Monoclonal (aa1-14) (2A2.1) Antibody, Application: IHC, ICC, IF, WB, ELISA, Reactivity: Xenopus, Format: Unconjugated, Unmodified, Descriptio: Beta Actin antibody RW-C20276 is an unconjugated mouse monoclonal antibody to xenopus Beta Actin (ACTB) (aa1-14). Validated for ELISA, ICC, IF, IHC and WB., Targe: Xenopus ACTB / Beta Actin, Synonym: ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1, Hos: Mouse, Reactivit: Xenopus (tested or 100% immunogen sequence identity), Clonalit: IgG1 Monoclonal, Clon: 2A2.1, Conjugation: Unconjugated, Purificatio: Ascites, Modification: Unmodified, Immunoge: Synthetic peptide corresponding to aa1-14 [DDDIAALVIDNGSGL] of Xenopus laevis beta Actin (KLH). Epitope: N-Terminus of beta isoform of actin. Molecular Weight: 42kD. Percent identity by BLAST analysis: Seq squirt, Sea anemone (93%); Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Medaka, Mosquito, Ant (87%)., Epitop: aa1-14, Specificit: Recognizes beta Actin in a wide variety of tissues and species. Does not cross-react with adult cardiac or skeletal muscle or amoeba beta actin. Species cross-reactivity: Human, bovine, Xenopus, Drosophila melanogaster, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp and goldfish) and leech., Application: IHC
ICC
Immunofluorescence
Western blot (1:2500)
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunofluorescence. Western Blot:1:2500. Useful as a loading control for a wide variety of cell extracts. Immunocytochemistry (acetone-fixed): 1:500. Immunohistochemistry: The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. B5, Methacarn, ethanol or Bouin's solution may also be used as fixatives. Immunofluorescence: Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque. Positive control: Cultured human or chicken fibroblasts., Presentatio: 15 mM sodium azide, 40% glycerol., Storag: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ACTB / Beta Acti: Uniprot: P60709 NCBI: NM_001101 NP_001092.1