Mouse HSPG ELISA Kit

Intended Uses:This HSPG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HSPG. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: HSPG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSPG antibody and an HSPG-HRP conjugate. The assay sample and buffer are incubated together with HSPG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to theHSPG concentration since HSPG from samples and HSPG-HRP conjugate compete for the anti-HSPG antibody binding site. Since the number of sites is limited, as more sites are occupied by HSPG from the sample, fewer sites are left to bind HSPG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSPG concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: HSPG Uniprot AC: Intended Use: Mouse HSPG ELISA Kitallows for the in vitro quantitative determination of HSPG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.