Mouse Cited2 ELISA Kit

Intended Uses: This CITED2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CITED2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay:CITED2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CITED2 antibody and an CITED2-HRP conjugate. The assay sample and buffer are incubated together with CITED2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CITED2 concentration since CITED2 from samples and CITED2-HRP conjugate compete for the anti-CITED2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CITED2 from the sample, fewer sites are left to bind CITED2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CITED2 concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Cited2 Uniprot AC: O35740; O35741; O35742; O35743; O55198; Q6PGA9; Intended Use: Mouse Cited2 ELISA Kitallows for the in vitro quantitative determination of Cited2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.