Mouse Wnt4 ELISA Kit

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT4 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Wnt4 Uniprot AC: P22724; Intended Use: Mouse Wnt4 ELISA Kitallows for the in vitro quantitative determination of Wnt4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.