Mouse TNF- Alpha ELISA Kit

Intended Use The mouse TNF-? ELISA is an enzyme-linked immunosorbent assay for the detection of mouse TNF-?. The mouse TNF-? ELISA is for research use only. Not for use in diagnostic procedures.. Principle of the Test An anti-mouse TNF-? coating antibody is adsorbed onto microwells. Mouse TNF-? present in the sample orstandard binds to antibodies adsorbed to the microwells. Following incubation unbound biological components are removed during a wash step and a biotin-conjugated anti-mouse TNF-? antibody is added and binds to mouse TNF-? captured by the first antibody. Following incubation unbound biotinconjugated anti-mouse TNF-? antibody is removed during a wash step. Streptavidin- HRP is added and binds to the biotinconjugated anti-mouse TNF-? antibody. Following incubation unbound Streptavidin- HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of mouse TNF-? present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 mouse TNF-? standard dilutions and mouse TNF-? sample concentration determined. Species : Mouse Storage: For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃. Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: TNF- Alpha Uniprot AC: Intended Use: Mouse TNF- Alpha ELISA Kitallows for the in vitro quantitative determination of TNF- Alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.