Mouse Thy1 ELISA Kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for THY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any THY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for THY1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of THY1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Thy1 Uniprot AC: P01831; Intended Use: Mouse Thy1 ELISA Kitallows for the in vitro quantitative determination of Thy1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.